Journal: Indian Journal of Experimental Biology

Article Title: Apoptotic and antiproliferative effects of Urtica dioica L. extract on K562 chronic myeloid leukemia cell line

doi: 10.56042/ijeb.v61i11.1574

Figure Lengend Snippet: Fig. 5 — (A) Representative immunoblot image; and (B) densitometric analysis showing cleaved PARP protein expression in K562 cells. The Western Blot analyse was carried out using primary antibody for (89 kD)-cleaved-PARP fragment after treatment with Urtica dioica extract at the end of 24 h incubation. β-actin protein was used as the loading control.

Article Snippet: Primary antibodies used in the study were β-actin (Novus Biologicals, NB600-501), Bax (SPM336, NBP2-32809), Bcl-2 (100/D5, NBP2-15200) and cleaved PARP (194C1439, NBP2-27335) monoclonal antibodies produced in mouse (Novus Biologicals).

Techniques: Western Blot, Expressing, Incubation, Control

Journal: Nature Communications

Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia

doi: 10.1038/s41467-022-30396-3

Figure Lengend Snippet: Panel of antibodies used for CyTOF staining.

Article Snippet: 143 Nd , PARP, cleaved , F21-852 , DVS-Fluidigm , 3143011A , YES.

Techniques: Staining, Marker

Journal: The Journal of Pathology

Article Title: Impaired decidual natural killer cell regulation of vascular remodelling in early human pregnancies with high uterine artery resistance

doi: 10.1002/path.4057

Figure Lengend Snippet: dNK cells induce vascular cell apoptosis. SGVSM-9 or SGHEC-7 cells were co-cultured with normal-RI dNK cells from individual patients in a dNK : vascular cell ratio of 1:3 and monitored over 50 h by time-lapse microscopy. Kinetics of VSMC apoptosis (A) and EC apoptosis (B) induced by normal-RI dNK cells. SGVSM-9 or SGHEC-7 cells were co-cultured alone (control) or with normal-RI dNK cells + /− 50 µ m zVAD-fmk over 50 h, and area under the kinetics curve data were generated for VSMC apoptosis (C) and EC apoptosis (D). Results are mean ± SEM of three separate experiments carried out in duplicate with 40 cells analysed per sequence. * p < 0.05; ** p < 0.001; *** p < 0.0001. (E) Apoptosis was further confirmed by western blot analysis of cleaved PARP (85 kDa) or cleaved caspase 3 (17/19 kDa) in SGVSM-9 or SGHEC-7 cells cultured with normal-RI dNK cells in a dNK : vascular cell ratio of 1:3 for 30 h. GAPDH (37 kDa) or tubulin (55 kDa) was detected as loading control

Article Snippet: After blocking for 1 h, the membrane was incubated with rabbit anti-human cleaved PARP (1/5000; G74A; Promega, Southampton, UK) or anti-cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or mouse anti-human tubulin (1/10 000; ab7291; Abcam, Cambridge, UK) overnight at 4 °C.

Techniques: Cell Culture, Time-lapse Microscopy, Control, Generated, Sequencing, Western Blot

Journal: The Journal of Pathology

Article Title: Impaired decidual natural killer cell regulation of vascular remodelling in early human pregnancies with high uterine artery resistance

doi: 10.1002/path.4057

Figure Lengend Snippet: High-RI dNK and NK92 cells do not induce vascular cell apoptosis. SGVSM-9 or SGHEC-7 cells were co-cultured with high-RI dNK cells from individual patients or NK92 cells in a NK : vascular cell ratio of 1:3 and monitored over 50 h by time-lapse microscopy. Results are mean ± SEM of experiments carried out with five separate dNK isolates in duplicate with 40 cells analysed per sequence. VSMC apoptosis (A) or EC apoptosis (B) after 50 h co-culture with high-RI dNK cells. Western blot analysis of cleaved PARP (85 kDa) in SGVSM-9 (C) or SGHEC-7 cells (D) cultured with normal-RI or high-RI dNK cells in a dNK : vascular cell ratio of 1:3 for 30 h. GAPDH (37 kDa) was detected as loading control. VSMC apoptosis (E) and EC apoptosis (F) compared between normal- and high-RI dNK cells. *** p < 0.0001; n = 5 separate experiments for each (normal-RI dNK cells were from additional experiments to those shown in ). The median gestational age of the samples used to generate dNK cells for VSMC co-culture (E) was 12.2 weeks (range 10.1–13.3 weeks) for normal-RI and 10.7 weeks (range 9.3–12.6 weeks) for high-RI ( p = 0.3, t -test). The median gestational age of the samples used to generate dNK cells for EC co-culture (F) was 11.4 weeks (range 9.4–12.7 weeks) for normal-RI and 10.4 weeks (range 9.1–11.7 weeks) for high-RI ( p = 0.6, t -test). VSMC apoptosis (G) or EC apoptosis (H) after 50 h co-culture with NK92 cells; experiments were repeated 3–4 times

Article Snippet: After blocking for 1 h, the membrane was incubated with rabbit anti-human cleaved PARP (1/5000; G74A; Promega, Southampton, UK) or anti-cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or mouse anti-human tubulin (1/10 000; ab7291; Abcam, Cambridge, UK) overnight at 4 °C.

Techniques: Cell Culture, Time-lapse Microscopy, Sequencing, Co-Culture Assay, Western Blot, Control